@article{J_S_Mithun K Rajan_Benitta Soosan Thomas_S_Vadivelan G_2022, title={Evaluation of antimicrobial and antioxidant activity of cleome gynandra l. and assess its anticancer activity against in vitro cancer model}, volume={9}, url={https://krjournal.com/index.php/krj/article/view/555}, DOI={10.26524/krj.2022.13}, abstractNote={<p><em>Cleome gynandra</em> Linn. is the native herb distributed at tropical and sub-tropical temperature. In this study, the antioxidant, antimicrobial and anticancer properties of the ethanolic extract of <em>Cleome gynandra</em> leaves were investigated also the anti-cancer activity was assessed against A549 lung cancer cell lines. The antioxidant activities were evaluated by 1,1‑diphenyl‑2‑picrylhydrazyl&nbsp; free radical scavenging activity. The IC<sub>50</sub> value of DPPH was ranged from 65.67 µg/ml for the leaf extract. The anti-microbial activity was showed against <em>Escherichia coli</em>, <em>Pseudomonas aeruginosa</em>, <em>Klebsiella pneumonia</em> and <em>Salomonella typhi</em>. The cytotoxic study was conducted and the results exhibited the strongest inhibitory activity against A549 lung cancer cell line as concentration dependent manner. The cytotoxic activity of the leaf extract was supported by the induction of apoptosis cell. The other assays to evaluate anticancer activity are DAPI (4,6 – diamminodino-2-phenylindole), PI (propidium iodide) and EtBr/AO (Ethidium bromide/acridine orange) staining methods. All the study results revealed the efficient anticancer activity of the ethanolic leaf extract of <em>Cleome gynandra </em>leaves<strong>. </strong>Thus, the herbal drug treatment might highly be recommended to treat effectively lung cancer cell line as an ideal choice or combinational therapy.</p&gt;}, number={2}, journal={Kongunadu Research Journal}, author={J, Abitharani and S, Sujith and Mithun K Rajan and Benitta Soosan Thomas and S, Viswanathan and Vadivelan G}, year={2022}, month={Dec.}, pages={1 - 8} }