Kongunadu Research Journal
Vol. 2 No. 2 (2015): Vol 2, Iss 2, Year 2015
Articles

INDUCTION OF CALLOGENESIS AND SHOOT REGENERATION OF A MEDICINAL PLANT SPECIES PERISTROPHE BICALYCULATA (RETZ.) NEES.

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  • Femila Jose V,
  • Asir Selin Kumar R
Femila Jose V
PG and Research Department of Botany, Scott Christian College, Nagercoil - 629 003, Tamilnadu, India
Asir Selin Kumar R
PG and Research Department of Botany, Scott Christian College, Nagercoil - 629 003, Tamilnadu, India
DOI: https://doi.org/10.26524/krj95
Published December 30, 2015
Keywords
  • Peristrophe bicalyculata, MS medium, multiple shooting, Acclimatization
How to Cite
V, F. J., & R, A. S. K. (2015). INDUCTION OF CALLOGENESIS AND SHOOT REGENERATION OF A MEDICINAL PLANT SPECIES PERISTROPHE BICALYCULATA (RETZ.) NEES. Kongunadu Research Journal, 2(2), 55-59. https://doi.org/10.26524/krj95

Abstract

In the present study, protocol for callus induction and regeneration for the medicinal plant species, Peristrophe bicalyculata (Retz.)Nees has been developed by using leaf explants. Young apical leaf explant was used for callus induction on MS medium containing BAP and NAA at 2`0 and 0.8 mgl-1 respectively showed
maximum callus induction (80%). The amount of callus responded for shoot formation (81%) was obtained in the MS medium containing BAP (2.0 mgl-1) and GA3 (0.3mgl-1).The elongated shoots were rooted on half strength medium supplemented with IBA (2.0 mgl-1) and IAA (0.2 mgl-1) for shoots rooted. Regenerated
plantlet were successfully acclimatized and hardened off inside the culture and then transferred to green Peristrophe bicalyculata, MS medium, multiple shooting, Acclimatizationhouse with better survival rate

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