- Cassia fistula, DPPH, ABTS , FRAP, Hydrogen peroxide and hydroxyl radical.
Abstract
The agro-climatic and traditional atmosphere of India provides a vital deposit of various herbs available in many parts of the country which are used as traditional medicine in Ayurveda, Unani, Siddha and Homeopathy. The over dosage and irregular practice of consuming various antibiotics has produced many
side effects and also helped in evolving multidrug resistant organisms. In this research we have focused on the usage of bark extracts of Cassia fistula (L.) for evaluating their antioxidant scavenging activity. With the traditional practice of shade drying and cold percolation method, the methanolic bark extract was chosen for various antioxidant activity like DPPH, ABTS+, FRAP, Hydrogen peroxide and hydroxyl radical scavenging activity. The plant extract demonstrated higher activity and IC50 value for extract in all the tests showed nearly 60μg/ml. The graphical representation also correlated with the spectrum analysis when plotted using the percentage of inhibition scavenging activity vs the concentration taken for each plant extracts.