EFFECT OF ANANUS COMOSUS LINN. PEEL EXTRACT ON 7, 12 DIMETHYL BENZ(Α)ANTHRACENE (DMBA) INDUCED MAMMARY CARCINOMA IN FEMALE SPRAGUE DAWLEY RATS

Abstract

Breast cancer is one of the leading second most prevalent malignant tumors in the world among women and its incidence continue to increase every year. Oxidative stress plays a key role in many cancers including breast cancer, oral cancer etc. Hence the use of natural antioxidants in day to day life is very
important privilege to quenching those diseases caused by free radicals. Therefore the present study was undertaken to evaluate the effect of Ananus comosus linn. peel extract on hepatic and renal expression of oxidative stress enzymes in 7, 12 dimethyl benz(α)anthracene induced mammary carcinoma. Female Sprague Dawley rats weighing 180 ± 10g were used for experimental purpose. The animals were divided in to five groups, group I served as control, group II animals were treated 7, 12-dimethyl-benz[a]anthracene, group III and group IV animals were treated with ethanolic extract of Ananus comosus (250 mg/kg body weight) and standard drug tamoxifen (10 mg/kg body weight) for 30 days after 90 days of tumor induction, group V animals were treated with ethanolic extract of Ananus comosus alone (250 mg/kg body weight) for 30 days. After the experimental period, the animals were sacrificed by cervical decapitation. Hepatic and renal tissues were used to analyze for protein, enzymic and non enzymic antioxidants levels. Rats treated with plant extract showed significant increased in antioxidant levels when compared to cancer bearing untreated rats. In conclusion, it can be inferred that ethanolic extract of Ananus comosus peel positively modulated the antioxidant activity by quenching and detoxifying the free radicals induced by 7, 12-dimethylbenz(α)anthracene in both liver and kidney tissues.