Vol. 4 No. 2 (2017): Vol 4, Iss 2, Year 2017 (Dr.S.Paulsamy Commemoration Issue)
Articles

IN VITRO REGENERATION AND MASS PROPAGATION OF AZIMA TETRACANTHA [LAM.] FROM THE LEAF EXPLANTS THROUGH CALLUS CULTURE

Mallika Devi T
PG and Research Department of Botany, Kandaswamy Kandar’s College, P. Velur, Namakkal – 638 182.
Published December 30, 2017
Keywords
  • Azima tetracantha, MS medium, Growth hormones, Acclimatization.
How to Cite
T, M. D. (2017). IN VITRO REGENERATION AND MASS PROPAGATION OF AZIMA TETRACANTHA [LAM.] FROM THE LEAF EXPLANTS THROUGH CALLUS CULTURE. Kongunadu Research Journal, 4(2), 52-56. https://doi.org/10.26524/krj202

Abstract

In the present study the protocol for callus induction and regeneration in Azima tetracantha has been developed in culture medium. The young apical leaf explants were used for callus induction on MS medium containing BAP and NAA at 1.0 and 0.4mgl-1 respectively showed maximum callus induction (73%). The amount of callus responded for shoot formation (74%) was obtained in the MS medium containing BAP (1.5 mgl-1) and NAA (0.3mgl-1).The elongated shoots were rooted on half strength medium supplemented with IBA (1.5 mgl-1) and Kn (0.4 mgl-1) for shoots rooted. Regenerated plantlets were successfully acclimatized and hardened off inside the culture and then transferred to green house with better survival rate.

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