Kongunadu Research Journal
Vol. 1 No. 1 (2014): Vol 1, Iss 1, Year 2014
Articles

ASYMBIOTIC SEED GERMINATION AND SYNTHETIC SEED PREPARATION OF VANDA TESTACEA (LINDL.) RCHB.F

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  • Thirugnanasampandan R,
  • Monisha S,
  • Gogul Ramnath M,
  • Ramya G
Thirugnanasampandan R
PG and Research Department of Biotechnology, Kongunadu Arts and Science College, Coimbatore.
Monisha S
PG and Research Department of Biotechnology, Kongunadu Arts and Science College, Coimbatore.
Gogul Ramnath M
PG and Research Department of Biotechnology, Kongunadu Arts and Science College, Coimbatore.
Ramya G
PG and Research Department of Biotechnology, Kongunadu Arts and Science College, Coimbatore.
DOI: https://doi.org/10.26524/krj23
Published June 30, 2014
Keywords
  • Vanda testacea,
  • seed germination,
  • artificial seed preparation,
  • Protocorm
How to Cite
R, T., S, M., M, G. R., & G, R. (2014). ASYMBIOTIC SEED GERMINATION AND SYNTHETIC SEED PREPARATION OF VANDA TESTACEA (LINDL.) RCHB.F. Kongunadu Research Journal, 1(1), 108-110. https://doi.org/10.26524/krj23

Abstract

A protocol for asymbiotic seed germination and synthetic seed preparation of Vanda testacea(Lindl.) Rchb.f. from mature pods was developed. Seed germination was successfully established on half strength B5 (Gamborg’s medium) supplemented with 1.0mg/L GA3. Protocorm like bodies (PLB’s) were formed after 90
days of culture and further artificial seed preparation of matured protocorm was successful using 5% sodium alginate and 100mM calcium chloride. The beads were inoculated on half strength B5 medium supplemented with 1.0mg/L GA3.

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