Kongunadu Research Journal
Vol. 5 No. 1 (2018): Vol 5, Iss 1, Year 2018
Articles

PRELIMINARY PHYTOCHEMICAL SCREENING AND HPTLC FINGER PRINTING ANALYSIS OF TRADITIONAL MEDICINAL PLANT PUERARIA TUBEROSA (ROXB. EX WILLD.)DC

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  • Zereena Viji,
  • Paulsamy S
Zereena Viji
Department of Botany, NSS College, Nemmara, Palakkad - 678 508, Tamil Nadu, India.
Paulsamy S
Department of Botany, Kongunadu Arts and Science College, Coimbatore - 641 029, Tamil Nadu, India.
DOI: https://doi.org/10.26524/krj254
Published June 30, 2018
Keywords
  • Pueraria tuberosa, phytochemical analysis, HPTLC
How to Cite
Viji, Z., & S, P. (2018). PRELIMINARY PHYTOCHEMICAL SCREENING AND HPTLC FINGER PRINTING ANALYSIS OF TRADITIONAL MEDICINAL PLANT PUERARIA TUBEROSA (ROXB. EX WILLD.)DC. Kongunadu Research Journal, 5(1), 56-59. https://doi.org/10.26524/krj254

Abstract

The medicinal value of a plant lies in the phytoconstituents present in it. These phytochemical compounds form the base of modern drugs. The aim of the present study is to identify the phytochemical constituents present in the traditional medicinal plant Pueraria tuberosa and to develop HPTLC fingerprint profile of acetone extract. Preliminary phytochemical screening was done to identify the phytoconstituents and HPTLC studies were carried out. CAMAG make HPTLC system equipped with Linomat 5applicator, TLC scanner 3, Reprostar 3 and WINCATS-1.4.3 software were used. The present study revealed the presence of
carbohydrates, proteins alkaloids, flavonoids, saponins, phenols and tannins in various extracts. The HPTLC fingerprint analysis of acetone extract of Pueraria tuberosa showed 10 peaks at 254nm. The components with Rf values 0.05, 0.21 and 0.72 were predominant with the percentage area of 34.52, 16.16 and 10.10
respectively. The preliminary phytochemical analysis revealed the presence of various phytochemicals, which were confirmed by the HPTLC fingerprint profile

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