IN VITRO ANTIOXIDANT ACTIVITY OF AQUEOUS AND ETHANOL LEAF EXTRACTS OF RHINACANTHUS NASUTUS (LINN.) KURZ. (ACANTHACEAE)

Abstract

In the present research work was to examine the possible antioxidant activities of the aqueous and ethanol leaf extract of Rhinacanthus nasutus (Linn.) Kurz. DPPH, Hydroxyl radical scavenging and reducing power assays were employed. The results showed that the DPPH activity of aqueous and ethanol leaf extract at the dose of 50μg/ml has exhibited in 63.81±0.013 and 79.36±0.028 inhibition with an IC₅₀ value of 21.39 and 29.41μg/ml. The highest Hydroxyl radical scavenging activity showed aqueous and ethanol leaf extract at the dose of 50μg/ml has exhibited in 96.18±0.029 and 121.23±0.081 inhibition with an IC₅₀ values of 30.19and 41.39μg/ml, the reducing power assay aqueous and ethanol leaf extract showed the 0.59 and 0.71 absorption at 700 nm extract at the dose of 50μg/ml suggested that promising antioxidant activity of crude aqueous and ethanol extract could be used as a source of natural antioxidants of R. nasutus and needs further studies to bring new natural products into pharmaceutical industries.