Vol. 6 No. 2 (2019): Vol 6, Iss 2, Year 2019
Articles

Isolation And Characterization Of Gut Bacterial Species Of Earthworm Lampito Mauritii

Deepak Kumar Parhi
Department of Zoology, Kongunadu Arts and Science College, Coimbatore- 641 029, Tamil Nadu, India.
Sahana Yasmine S
Department of Zoology, Kongunadu Arts and Science College, Coimbatore- 641 029, Tamil Nadu, India.
Sridhar T
Department of Zoology, Kongunadu Arts and Science College, Coimbatore- 641 029, Tamil Nadu, India.
Deepthi M.P
Department of Zoology, Kongunadu Arts and Science College, Coimbatore- 641 029, Tamil Nadu, India.
Kathireswari P
Department of Zoology, Kongunadu Arts and Science College, Coimbatore- 641 029, Tamil Nadu, India.
Published December 30, 2019
Keywords
  • Anecic, Lampito mauritii, gut, microbes
How to Cite
Parhi, D. K., S, S. Y., T, S., M.P, D., & P, K. (2019). Isolation And Characterization Of Gut Bacterial Species Of Earthworm Lampito Mauritii. Kongunadu Research Journal, 6(2), 45-50. https://doi.org/10.26524/krj300

Abstract

Lampito mauriti is an Anecic earthworm living in the topsoil and it is geophytophagous in nature.This earthworm is an important soil macro fauna as it has the dual role of an ‘ecosystem engineer’ due to the ability to build burrows as well as ‘keystone species’ in soil food webs because of its function in degradation
of organic wastes. The present study investigates the gut of this earthworm to find the most predominant bacterium harbored inside. Gut contents were extracted and streaked on bacteriological media. The predominant type of colony was identified based on size, pigmentation, form, margin and elevation. Then
different colonies were isolated and streaked separately to get pure colonies. The bacteria was subjected to several biochemical tests like Motility test, Gram staining, Methyl Red test, Voges-Proskauer test, Indole production test, Carbohydrate Fermentation test, Catalase test and Starch Hydrolysis test in order to identify the species. The bacterial species identification was done using Bergey’s manual. The bacterial isolates such as Micrococcus spp, Veillonella spp, Bacillus spp and Streptococcus spp were identified.

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