- Extracellular products, Biocompatibility, Antagonistic activity, Haemolytic activity
Abstract
Sulphur is one of the essential plant nutrients and it contributes to yield and quality of crops. Thiobacillus play an important role in sulphur oxidation in soil and domestic wastewater.The present study is sought to understand the frequency of Thiobacillus in sewage water samples collected from hospital and domestic area.Distribution of sulphur oxidizing bacteria were analysed by using modified Thiobacilli agar. Five isolates were selected and analysed their selected putative virulence properties phenotypically.Also they were showed biocompatible within the group of the Thiobacilli.The extracellular products (culture supernatant) of Thiobacilli showed a substantial level of haemolytic activity and antagonistic activity. Among the isolates from sewage sources, TB-D1 and TB-D2 produced higher level of bacteriocin against pathogenic bacteria due to the formation of maximum inhibition zone. It reveals that the bacteriocin from Thiobacillus showed the elevated antagonistic activity against most of the pathogenic bacteria, as we tested.The strains TB-D4 and TB-D5 showed beta and alpha haemolysis, it might have virulent properties and cause infection in human and animals.The strain TB-D5 showed the maximum inhibitions while compared with others. It is visibly showing presence of virulence factors in the extracellular metabolites of Thiobacilli.